Number Of Phytochemical Compounds Research Articles

A Review on Phytochemical Constituents and Pharmacological Activities of Buchanania lanzan Sprang., Millettia peguenensis Ali., Evolvulus alsinoides L.

In this study, Buchanania lanzan sprang.,Millettia peguenensis Ali., and Evolvulus alsinoides L. were analyzed in terms of phytochemical composition, distribution, and ethnomedicinal uses. These plants are used in Ayurveda, Unani, and Chinese medicine as ethnomedicine. Plants have numerous properties, such as antioxidants, antibiotics, anti-bacterial, anti-ulcer, anti-inflammatory, antistress, and antidiabetic. In this review, we intend to demonstrate the phytochemical constituents, as well as the traditional medicinal uses of these herbs. Based on a comprehensive review of various research papers and literature available on these three plants, as well as the present research done by us, we have tried to compile information regarding Buchanania lanzan sprang.,Millettia peguenensis Ali., and Evolvulus alsinoides L.. Various scientific databases were used to find information on the anti-diabetic properties of B. lanzan, M. peguensis, and E. alsinodes, like Scopus, PubMed, ScienceDirect, and Google Scholar. Different parts of the plants were examined for their phytochemical, toxicological, antioxidant, antidiabetic, antiinflammatory, antistress, and many more. They contain a number of phytochemical compounds, including flavonoids, saponins, alkaloids, tannins, terpenes, and glycosides, which are responsible for their ability to treat diabetes, wounds, and oxidative stress. A crude extract of the different parts has valuable bioactive properties and could have ethnopharmacological relevance for managing different kinds of diseases. These phytoconstituents can be used in the formation of drugs.

Phytochemical Phytochemical Compounds and Antihyperglycemic Activities from Beverage Composed Pluchea indica Less-Black Tea-Honey

Pluchea indica Less has been developed into tea bag because it contains a number of phytochemical compounds, such as lignin, terpenes, benzoids, phenylpropanoids, alkanes, saponins, catechins, alkaloids, tannins, sterols, phenolhydroquinones, and flavonoids, which have been shown to have several biological activities, such as antioxidants, anti-inflammatory, anticholesterol, and antihyperglycemic. In its development, pluchea tea can be combined with black tea to produce certain functional values. Consumption of pluchea-black tea in daily life can be done with the addition of lemon or honey to increase antihyperglycemic activity. However, this study focused on determining the profile of phytochemical compounds and antihyperglycemic activity of pluchea-black tea honey drink. The research design used was a Randomized Block Design (RAK) with one factor including honey concentration consisting of six treatment levels: 0, 1, 2, 3, 4 and 5% (v/v). Each treatment was repeated four times. Parameters carried out are the profile of phytochemical compounds and antihyperglycemic activity through in vitro analysis, namely the activity of inhibiting a-amylase and a-glucosidase enzymes. The results showed that the use of honey in the manufacture of pluchea-black tea had an effect on the profile of phytochemical compounds and antihyperglycemic activity. The use of honey with a concentration of 5% produced the highest antihyperglycemic activity, 73.21±3.57% for the ability to inhibit the alpha-amylase enzyme and 77.27±5.25% for the ability to inhibit the alpha-glucosidase enzyme, respectively

Analysis of Phytochemical compounds present in some Traditional Anti-dermatophytic plants by Thin Layer, Column Chromatographic Methods

Traditionally, various medicinal plants were known to possess anti-dermatophytic potential and have been used for many years to treat skin diseases. We are aimed to examine anti-dermatophytic plants like Calotropis gigantea (L.) R. Br., Cassia alata (L) Roxb., Kaempferia galanga L., Melia azedarach L. and Sesbania grandiflora (L) Pers. for the quantification and separation of phyto-chemical compounds through thin layer and column chromatography. Five plant samples comprising of leaves, stem fiber and rhizome were separately extracted using five solvents such as, ethanol, methanol, acetone, chloroform and petroleum ether. The solvent extracts were subsequently investigated for qualitative, quantitative phytochemical, thin layer and column chromatographic analysis using standard assays. The experimental findings revealed the varied presence of compounds such as sugar, amino acid, protein, phenol, alkaloid and flavonoid in the solvent extracts used. In TLC profiling, a number of phyto chemical compounds, at different ranges of visible spots, with different Rf values at varying solvent system, were identified. Also, column fractions at different solvent run ratio (hexane: acetone = 8:2, 6:4, 4:6, 2:8) were identified with a wide range of color variations at different retention time and were collected sequentially. The present analysis exhibit a significant role to identify and differentiate therapeutic phyto-compounds which helps for further study on isolation and characterization with anti-dermatophytic potential.

Bitterness reduction of Green Chiretta (Andrographis paniculata) leaves and its functionality

Green chiretta leaves, known for its bitterness, is an Indonesian native traditional herb, used for its functional and medicinal properties. This research aimed to reduce the bitterness of green chiretta leaves using adsorbent technique and to observe how it might affect the functional properties of the chiretta leaves. The debittering process was done by utilizing diatomite, bentonite, and attapulgite as the adsorbent, together with 5, 10, and 20 minutes of boiling time. The leaves were then subjected to bitterness and phytochemical analysis. The bitterness was analyzed using scalar methods, the antioxidant properties were analyzed by measuring the IC50 value against DPPH solution, and the phytochemical compounds (total phenolic, flavonoid, alkaloid, tannin, and terpenoid) was analyzed quantitatively. Different adsorbent will adsorb different phytochemical compound. As the boiling time increases, the number of phytochemical compounds also reduced, hence resulting in a decrease of bitterness and IC50 value. This study found that the use of attapulgite with 20 minutes of boiling reduce the bitterness up to (90%) of the leaves due to its ability to adsorb terpenoid content while still retained their antioxidant properties better than bentonite or diatomite.

The Effect of Ginkgo biloba Extracts on Candida albicans Isolated from Healthy Persons

The objective was to study the effect of prepared ginkgo biloba extracts against Candida albicans isolated from healthy persons. Conducting susceptibility test, biofilm formation test, phytochemical screening test, and antioxidant activity test. One hundred oral swabs sample were obtained from healthy persons with oral lesion attending dentistry teaching hospital in dentistry college, their age ranged from 1-30 years of both sexex. The studied samples collected through 8 months (April - December / 2018). This study included two different types of ginkgo bilola extracts were prepared as aqueous and ethanolic extracts. Many tests were used, which included isolation and identification of C.albicans, conduct susceptibility test, biofilm formation test, phytochemical screening test, and antioxidant activity test for both aqueous and ethanol ginkgo biloba extracts. From 100 healthy person involved in this study, there were 21(21%) C. albicans isolates revealed from clinical specimens. Aqueous and ethanol ginkgo biloba extracts were used to study their effects against C.albicans. Zone of inhibition was higher in ethanol than aqueous extracts. Three 3 (15%) isolates showed positive biofilm formation in tube method, phytochemical reaction in ethanol extract showed 5 phytochemical compounds, while aqueous extract showed 4 phytochemical compounds, in addition to antioxidant activity in ethanol extract was higher than aqueous. In conclusion C. albicans is the only species from genus Candida isolated from oral lesion in this study, ethanol ginkgo biloba extract have a good antifungal activity, higher number of phytochemical compounds and a higher antioxidant activity than aqueous extract.

The Effect of Ginkgo biloba Extracts on Candida albicans Isolated from Healthy Persons

The objective was to study the effect of prepared ginkgo biloba extracts against Candida albicans isolated from healthy persons. Conducting susceptibility test, biofilm formation test, phytochemical screening test, and antioxidant activity test. One hundred oral swabs sample were obtained from healthy persons with oral lesion attending dentistry teaching hospital in dentistry college, their age ranged from 1-30 years of both sexex. The studied samples collected through 8 months (April - December / 2018). This study included two different types of ginkgo bilola extracts were prepared as aqueous and ethanolic extracts. Many tests were used, which included isolation and identification of C.albicans, conduct susceptibility test, biofilm formation test, phytochemical screening test, and antioxidant activity test for both aqueous and ethanol ginkgo biloba extracts. From 100 healthy person involved in this study, there were 21(21%) C. albicans isolates revealed from clinical specimens. Aqueous and ethanol ginkgo biloba extracts were used to study their effects against C.albicans. Zone of inhibition was higher in ethanol than aqueous extracts. Three 3 (15%) isolates showed positive biofilm formation in tube method, phytochemical reaction in ethanol extract showed 5 phytochemical compounds, while aqueous extract showed 4 phytochemical compounds, in addition to antioxidant activity in ethanol extract was higher than aqueous. In conclusion C. albicans is the only species from genus Candida isolated from oral lesion in this study, ethanol ginkgo biloba extract have a good antifungal activity, higher number of phytochemical compounds and a higher antioxidant activity than aqueous extract.

Pooja Bhatt and Niki Nautiyal

Abstract-Tinosporacordifoliaisapopularmedicinal plant which is used in several traditional medicines to cure various diseases. The common names are Amrita and Guduchi, belonging to the family of Menispermaceae. The aim of the study was to study the stem extract of traditional medicinal plant, T. cordifolia for qualitative estimation of phytoconsituents and subsequently to determine its antibacterialactivityagainsttwotestmicroorganisms Escherichia coliand Pseudomonas aeruginosato authenticate its use in traditional medicines. Stems were air-dried and coarsely powdered samples were subjectedto Soxhlet extractionusingdiversesolvents(Hexane,chloroform, ethyl acetate and methanol and water). Freshly prepared extracts were exposed to standard phytochemical analysis for qualitative estimation of phytoconsituents. The antibacterial activity of the stem extract of T. cordifolia was determined by agar well diffusion method. Phytochemical analysis revealed the presence of several phytochemicals viz., alkaloids, flavonoids, steroids, phenol, tannins, steroid, terpenoids, saponins and sugars. The methanolic extract displayed the presence of highest number of phytochemical compounds. It may be due to the higher solubility of active components in this solvent as compared to other solvents. The results revealed that the methanolic extract exhibit the effective antibacterial activity against the tested bacterial species . The studies justify the use of T. cordifolia in traditional medicines. The investigation further proposed that the metabolites present in leaf tissue of T. cordifolia can be potential source of novel natural antibacterial and antioxidant agents and can be prospective applications in food industry as an antioxidant

Hydroxycinnamic acid derivatives isolated from hempseed and their effects on central nervous system enzymes

New neuroprotective treatments of natural origin are being investigated. Both, plant extracts and isolated compounds have shown bioactive effects. Hempseed is known for its composition of fatty acids, proteins, fibre, vitamins, as well as a large number of phytochemical compounds. After a defatting process of the seeds, hydroxycinnamic acids and its amine derivatives are the majoritarian compounds in an ethyl acetate fraction (EAF). In the present study, we investigated in vitro effect on neuronal enzymes: MAO-A, MAO-B, tyrosinase and acetylcholinesterase. Besides, the effect of EAF on striatal biogenic amines in mice was evaluated. Both, EAF and isolated compounds (N-trans-caffeoyltyramine and N-trans-coumaroyltyramine), showed inhibitory action on MAO-A, MAO-B and tyrosinase. Furthermore, an increasing of biogenic amines was observed in the corpus striatum of the mice, after administration of EAF. These findings show that EAF and the hydroxycinnamic acid derivatives may represent a potential treatment in degenerative neuronal diseases.

Investigation of Brassica juncea, Forsythia suspensa, and Inula britannica: phytochemical properties, antiviral effects, and safety

BackgroundGeneral antiviral agents such as oseltamivir are associated with certain adverse effects and the emergence of resistance. This study investigated the phytochemical properties, antiviral activities, and safety of three herbs used in traditional Korean medicine.MethodsExtracts of three medicinal herbs (Brassica juncea, Forsythia suspensa, and Inula britannica) were prepared using ethanol or water. The total phenolic, flavonoid, and saponin content, condensed tannin content, and reducing sugar content of the herb extracts were determined via phytochemical screening. Tandem mass analysis was performed using an ultra-performance liquid chromatography (UPLC)-electrospray ionization (ESI)-Q/Orbitrap instrument. Virus titrations were determined via tissue culture infective dose (TCID50) and cytotoxicity assays. Hemolysis and hepatotoxicity were measured to determine safety.ResultsAmong the three medicinal herbs, F. suspensa showed the highest concentration of phenolic compounds, flavonoids, and saponins. The number of phytochemical compounds detected via tandem mass analysis of B. juncea, F. suspensa, and I. britannica was 5 (including sinigrin, m/z [M-H] = 358.02), 14 (including forsythoside A, m/z [M-H] = 623.19), and 18 (including chlorogenic acid, m/z [M-H] = 353.20), respectively. The antiviral effects of the B. juncea extracts (ethanol and water) and I. britannica extract (ethanol) were further investigated. The ethanol extract of B. juncea showed a 3 Log TCID50/25 μL virus titration reduction and the water extract showed a selectivity index of 13.668 against infected influenza H1N1 virus A/NWS/33. The B. juncea extracts did not show hemolysis activities and hepatotoxicity (< 20%). The ethanol extract of I. britannica showed the most effective virus titration decrease, whereas its hemolytic and hepatotoxicity values were the most significantly different compared to the control. Despite the high concentration of phytochemicals detected in F. suspensa, the extract showed approximately 1 Log TCID50/25 μL at the highest concentration.ConclusionB. juncea may show antiviral effects against H1N1 in a host. In addition, B. juncea may also show decreased disadvantages compared to other antiviral agents.

The anticancer, antioxidant and phytochemical screening of Philenoptera violacea and Xanthocercis zambesiaca leaf, flower & twig extracts

The aim of this study was to investigate the antioxidant potential, anticancer activity, phytochemical constituents and total phenolic content of Philenoptera violacea and Xanthocercis zambesiaca leaves, flower & twig extracts. The anticancer activity of the extracts was tested in the 5-cell line panel consisting of MCF7 (breast cancer), HCT116 (colon cancer), TK10 (renal), UACC62 (melanoma) and PC3 (prostate cancer) by Sulforhodamine B (SRB) assay at the CSIR. The scavenging activities of the Xanthocercis zambesiaca extract and Philenoptera violacea was determined by 2, 2- diphenyl-1-picrylhydrazyl (DPPH) assay and was compared with standard antioxidant (ascorbic acid). Xanthocercis zambesiaca showed moderate (50%) free radical scavenging activity, while Philenoptera violacea extract had low free radical scavenging activity at the concentration of 2.5 mg/ml when compared to ascorbic acid. Qualitative phytochemical analysis of these plant extracts confirmed the presence of tannins, flavonoids, steroids, terpenoids, alkaloids and cardiac glycosides from Philenoptera violacea extract, while Xanthocercis zambesiaca extract showed the presence of flavonoids, saponnins, terpenoids and glycosides. Both extracts showed inactivity against all 5 cell lines with an IC 50 of more than 100 g/ml. Our results indicate that, Xanthocercis zambesiaca extract is a weak source of antioxidant and Philenoptera violacea extract has a number of phytochemical compounds. Future study will be done to isolate and identify the active compounds of both these plants.

CHARACTERISTICS OF JELLY CANDY OF PAPAYA (Carica papaya L.) WITH ADDITION OF COW GELATIN

Papaya is very potential to be developed as an industrial raw material candy because it contains a number of phytochemical compounds that are beneficial for the body. This study aims to investigate the characteristics of jelly candy of papaya with addition of cow gelatin by 6%, 8% , 11% and 13%. In jelly candy made observations on pH, moisture content, ash content, reducing sugar content, sucrose content, vitamin C content, total acid, antioxidant activity by DPPH, total plate count, organoleptic and the best jelly candy analysis of beta-carotene The results showed that the addition of 11% cow gelatin to produce the best characteristics of jelly candy. The parameters are pH 4.83, 17.63% moisture content, ash content of 0.98% , 19.75% reduction sugar , sucrose content of 35.48%, levels of vitamin C 59.81 mg/100gr material, total acid 2.09%, 35.33% antioxidant activity, total plate count 3.9 x 103 colonies/g, 7.9934 mg/100 g levels of beta-carotene, and organoleptic tests with a level, 95% color, scent 85%, 85% flavor, and texture of 95%.

CHARACTERISTICS OF JELLY CANDY OF PAPAYA (Carica papaya L.) WITH ADDITION OF COW GELATIN

Papaya is very potential to be developed as an industrial raw material candy because it contains a number of phytochemical compounds that are beneficial for the body. This study aims to investigate the characteristics of jelly candy of papaya with addition of cow gelatin by 6%, 8% , 11% and 13%. In jelly candy made observations on pH, moisture content, ash content, reducing sugar content, sucrose content, vitamin C content, total acid, antioxidant activity by DPPH, total plate count, organoleptic and the best jelly candy analysis of beta-carotene The results showed that the addition of 11% cow gelatin to produce the best characteristics of jelly candy. The parameters are pH 4.83, 17.63% moisture content, ash content of 0.98% , 19.75% reduction sugar , sucrose content of 35.48%, levels of vitamin C 59.81 mg/100gr material, total acid 2.09%, 35.33% antioxidant activity, total plate count 3.9 x 103 colonies/g, 7.9934 mg/100 g levels of beta-carotene, and organoleptic tests with a level, 95% color, scent 85%, 85% flavor, and texture of 95%.

A review on Decalepis hamiltonii Wight Arn.

Decalepis hamiltonii is a climbing shrub with aromatic tuberous roots distributed in Southern parts of Peninsular India. Its tuberous roots are widely used as a health drink and are well known for its medicinal properties. D. hamiltonii is one of the important plants in Ayurvedic system of medicine in India and are used in curing various diseases like stomach disorders, gastric ulcers and to stimulate appetite. This plant has been used in the preparation of several herbal drugs like Amrutamataka taila, Drakshadi churna, Shatavari rasayana and Yeshtimadhu taila. In the present review, traditional uses, uses as food and health drinks, phytochemistry, pharmacology and conservation of this endangered species are highlighted. A number of phytochemical compounds have been isolated from this plant; of these, 2-hydroxy-4-methoxy benzaldehyde (HMB) is an abundant aromatic bioactive compound with greater biological significance. The tubers have antimicrobial, antipyretic, antiulcer, antidiabetic, antioxidant, anti-inflammatory, chemoprotective, cytoprotective, insecticidal, neuroprotective and hepatoprotective activities. Natural seed germination is very low in this species, that is, 6% because of hard seed coat, less seed dormancy period and due to self-incompatibility. In vivo and in vitro conservation methods have been standardized to this endangered taxon by studying seed germination, field cultivation methods and by developing rapid micropropagation techniques. Among different plant growth regulators tested, 6-benzylaminopurine (BAP) played a significant role in shoot multiplication, whereas, indole-3- butyric acid (IBA) and silver nitrate (AgNO3) influence rooting efficiently. The review aims to provide sufficient baseline information for further works and commercial exploration.  Key words: Decalepis hamiltonii, phytochemistry pharmacology, conservation.